| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lunn, Emma K (13)
|
MASSC |
5:18.17 |
5:24.46 |
6.29 |
| 2 |
Gendzel, Maya C (13)
|
MASSC |
5:30.04 |
5:32.77 |
2.73 |
| 3 |
Ott, Madeleine F (14)
|
MASSC |
5:41.41 |
5:36.25 |
-5.16 |
| 4 |
Kubiak, Michelle D (13)
|
MARAYS |
5:42.30 |
5:48.53 |
6.23 |
| 5 |
Wilson, Mekhi S (14)
|
MASSC |
5:45.56 |
6:00.93 |
15.37 |
| 6 |
O'Shea, Isabelle (13)
|
MAUN-E |
6:09.23 |
6:01.46 |
-7.77 |
| 7 |
Kennedy, Kayleigh J (13)
|
MASSC |
6:13.09 |
6:07.42 |
-5.67 |
| 8 |
Scharschan, Clare J (14)
|
MASSC |
|
6:14.73 |
- |
| 9 |
Cappola, Alexandra G (11)
|
MASSC |
|
6:18.43 |
- |
| 10 |
Galvin, Tamar J (14)
|
MAUN |
6:23.66 |
6:37.01 |
13.35 |
| 11 |
Ott, Cassidy M (11)
|
MASSC |
7:00.69 |
6:48.46 |
-12.23 |
|
Holtsberg, Cathryn F (14)
|
MASSC |
6:02.99 |
NS |
- |